• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Identification of genes up-regulated in dedifferentiating Nicotania glauca pith tissue, using an improved method for constructing a subtractive cDNA library.
【2h】

Identification of genes up-regulated in dedifferentiating Nicotania glauca pith tissue, using an improved method for constructing a subtractive cDNA library.

机译：使用构建消减cDNA文库的改进方法，鉴定去烟烟草青髓组织中上调的基因。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pith explants of Nicotiana glauca grown in vitro in synthetic medium supplemented with 2,4 dichlorophenoxyacetic acid (2, 4 D), are induced to dedifferentiate. Treatment with actinomycin D within the first 4-8 h of culture (but not later) is lethal and the explants die, implying a requirement for de novo transcription. The genes expressed during the initial period of culture are presumably critical for subsequent cell survival and proliferation, but so far their identity is unknown. We have constructed a subtractive cDNA library, enriched in sequences more abundant in dedifferentiating tissue than in pith. The subtractive library contains approximately seven major species, two of which, NGSUB7 and NGSUB8, are highly abundant. In Northern blots, these two hybridized to mRNA species whose abundance increased significantly but transiently during the first 4 to 8 h of culture. The sequence of NGSUB7 showed no significant homology at a nucleotide or derived amino acid level with any previously reported sequence. NGSUB8 however, showed significant homology over part of the derived amino acid sequence to several yeast and bacterial proteins with DNA binding function. We propose that the two recombinants represent transcripts from two novel genes edeA and edeB, which are expressed early in dedifferentiation.
机译：在补充有2,4二氯苯氧基乙酸（2，4 D）的合成培养基中体外生长的烟草白炭黑的外植体被诱导去分化。在培养的最初4至8小时内（但不晚于此）用放线菌素D处理是致命的，外植体死亡，这意味着需要从头转录。在培养初期表达的基因可能对随后的细胞存活和增殖至关重要，但到目前为止，它们的身份尚不清楚。我们已经构建了一个消减cDNA文库，该文库富含在去分化组织中比在髓中更丰富的序列。减性文库包含大约七个主要种类，其中NGSUB7和NGSUB8这两个种类非常丰富。在RNA印迹中，这两个与mRNA种类杂交，其mRNA种类在培养的前4至8小时内显着增加，但瞬时增加。 NGSUB7的序列在核苷酸或衍生的氨基酸水平上与任何先前报道的序列均无明显同源性。然而，NGSUB8在部分衍生的氨基酸序列上与具有DNA结合功能的几种酵母和细菌蛋白显示出显着的同源性。我们建议这两个重组代表来自两个新基因edeA和edeB的转录本，它们在去分化早期表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号